Cryopreservation of spermatozoa of indigenous stinging catfish, Heteropneustes fossilis (Bloch) for ex-situ conservation

Authors

  • MD. RAFIQUL ISLAM SARDER Bangladesh Agricultural University, Mymensingh
  • MD. ABUL KALAM AZAD
  • K.M. SHAHRIAR NAZRUL
  • MOHAMMAD RASHED

DOI:

https://doi.org/10.52168/bjf.2020.32.02

Keywords:

Cryopreservation, Spermatozoa, H. fossilis, Breeding

Abstract

An experiment was conducted to develop cryopreservation protocol for spermatozoa of stinging catfish, Heteropneustes fossilis and to use the cryopreserved sperm in its breeding trials. The activation of sperm motility at various concentrations of NaCl solution was tested and complete activation and inhibition of sperm were obtained at 0.4% and 0.9% to 1% NaCl solution respectively. In toxicity test, sperms were incubated with DMSO, methanol and ethanol at 5, 10, and 15% concentrations where DMSO and methanol produced better motility at 5 and 10% concentration with Alsever’s solution and egg-yolk citrate at 5 and- 10 min incubation period. Three extenders- Alsever’s solution, egg-yolk citrate and Ginsburg Fish Ringer solution and three cryoprotectants- DMSO, methanol and ethanol were used for cryopreservation of sperm, and among the diluents, Alsever’s solution with 10% DMSO showed best performance producing 77.50±3.22% post-thaw motility. On the other hand, egg-yolk citrate and Ginsburg Fish Ringer solution along with 10% DMSO produced 63.75±2.39% and 62.50±3.22% post-thaw motility, respectively. Sperm preserved with Alsever’s solution plus 10% DMSO produced 52.5±3.34% and 38.0±2.39%fertilization and hatching, and those preserved with Alsever’s solution plus 10% methanol produced 46.9±3.11% and 32.7±2.70% fertilization and hatching respectively. The fry produced using cryopreserved and fresh sperm grew well and no significant difference (p>0.05) was found between two groups.

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Published

2020-07-02