Development of sperm cryopreservation technique of minor carp, bata (Labeo bata Ham. 1822) for ex-situ conservation

Authors

  • MIRZA NUSRAT NOOR
  • MD. RAFIQUL ISLAM SARDER
  • ANAS AL ISLAM
  • DURIN AKHTER JAHAN
  • MD. MOUDUD ISLAM

Keywords:

Labeo bata, Cryopreservation, Conservation

Abstract

The study was focused on the development of suitable sperm cryopreservation protocol
for minor carp, bata (Labeo bata). Sperms were collected from hormone induced males by
stripping. Sperm motility activation was assessed in different concentrations of activation solution
(NaCl) and observed that sperm motility diminished with increasing concentration of NaCl and
fully inhibited at 1.10% NaCl. Toxic effects of cryoprotectants to sperm was assessed using two
cryoprotectants- DMSO and methanol along with two extenders (Alsever’s solution and egg-yolk
citrate) at 5, 10 and 15% concentrations for 5 to 45 min. Alsever’s solution along with 10%
DMSO resulted in the best equilibration (86.66±3.33%) as well as post-thaw motility
(70.0±2.88%) while egg-yolk citrate with DMSO produced similar equilibration motility
(68.33±4.41%). In breeding trials, sperm preserved with Alsever’s solution and DMSO showed
highest fertilization rate (87.5±2.5%) and hatching rate (18.4±6.8%) while sperm preserved
with egg-yolk citrate and DMSO produced 82.5±2.5% fertilization and 16.4±5.8% hatching
rates. Fresh sperm yielded 92.5±2.5% and 63.6±6.6% fertilization and hatching rate
respectively. The protocols that have been standardized can be applied for preservation of sperm
of L. bata.

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Published

2019-08-22