Genetic diversity of mosquito fish(Gambusia affinis) in hatchery and wild populations

Abstract

The invasive mosquito fish Gambusia affinis, affected hatchery populations and abundant in
different wetlands of Jashore regions which carry ectoparasites. This study evaluated genetic diversity
between hatchery and wild populations of G. affinis using polymorphic SSR DNA markers, which have not
yet been clarified. A total of 50 polymorphic SSR and microsatellite alleles were identified by electrophoresis
with a mean value of polymorphic information content (PIC) of 0.94, highest PIC values (0.97) were found
for CytBand Gaaf15 marker, and lowest values (0.92) for Gaaf14, Gaaf22, and Gaaf23.A total of 50 (ranges
165-452bp) DNA fragments (alleles) were scored, of which 307 fragments were highly polymorphic with
cytBSSRmarker. A UPGM Adendrogram constructed based on Nei’s coefficient, generated by SSR DNA
marker showed very limited genetic variation. Genomic sequences of DNA fragments for each population
were found genetically closest with good adaptability in changing environmental scenario. The SSR marker
techniques are found to be the rapid and effective tool for diversity assessment, this result might be helpful to
the development of sterile G. affinis to control further reproduction.