Histological identification of gonadal maturation and induced breeding of tengra, Mystus vittatus (Bloch) for seed production

Abstract

The study was conducted toidentify gonadal maturation and develop artificial breeding
technique of striped dwarf catfish Mystus vittatus. Gonado-somatic index (GSI) was found to be
highest as 20.81±2.73 and 6.06±2.96 for female and male respectively in the month of July.The
mean fecundity was recorded between 13138±1365.94 and 25095.2±6792.5. Six developmental
stages of ovary viz. chromatin nucleolar stage, early and late perinucleolar stage, yolk vesicle
stage and early and late yolk granule stage; and four developmental stages of testis named
spermatogonia, spermatocytes, spermatids and spermatozoa were observed. For induced breeding
two inducing agents viz. PG extract and Flash (GnRHa) were used. Three different doses of PG
such as. 2 and 6 mg (T1); 4 and 8 mg (T2) and 6 and 10 mg (T3) per kg bodyweight of male and
female respectively were tested to standardize the PG dose.T1 provided best result in respect of
ovulation of females, fertilization and hatching rates of eggs. In case of Flash, three different
doses viz. 0.5 ml (T1), 1 ml (T2) and 2 ml (T3) per kg bodyweightwere used for both male and
female. The best result was obtained from T2 and no significant difference (p>0.05) in respect of
ovulation, fertilization and hatching rates of eggs was observed among the treatments.