Dietary probiotics enhance the immunity of Vietnami koi (Anabas testudineus) against Pseudomonas sp.

IMMUNITY OF A. TESTUDINEUS AGAINST PSEUDOMONAS SP.

Abstract

The present study evaluated the efficacy of dietary probiotic Biozyme on the immunological parameter and disease resistance against Pseudomonas sp. infection in Vietnam Koi (Anabas testudineus). Fish with an average body wt. 25±5 g were fed twice a day at 5% of their body wt.) experimental diet prepared with the supplementation of 0 g, 0.5 g, 1.0 g, 1.5 g and 2.0 g Biozyme probiotic per kg feed that enhanced the immune potentiality investigated on weeks 1, 2 and 4. The phagocytic and bactericidal activity significantly increased in A. testudineus fed with 2.0 g Biozyme probiotic per kg feed against Pseudomonas sp. on weeks 2 and 4. Further, the diet enriched with 2.0 g of Biozyme (probiotics) resulted in lowest mortality (20%) highest protection with 75% survival from Pseudomonas sp. infection than 0.5 g, 1.0 g and 1.5 g of addition with diet that resulted 46.67%, 40% and 33.33% mortality respectively. 2.0 g probiotic/kg of feed also showed highest growth compared to other dose. The results suggest that the dietary supplementation of 2.0 g probiotics/kg feed significantly (p< 0.05) reduce mortality, act as immunostimulants, and promoter and showed resistance in A. testudineus.
Keyword: Anabas testudineus, dietary probiotics, Pseudomonas sp